Aging-induced stem cell mutations as drivers for disease and cancer

Aging is characterized by a decrease in genome integrity, impaired organ maintenance, and an increased risk of cancer, which coincide with clonal dominance of expanded mutant stem and progenitor cell populations in aging tissues, such as the intestinal epithelium, the hematopoietic system, and the male germline. Here we discuss possible explanations for age-associated increases in the initiation and/or progression of mutant stem/progenitor clones and highlight the roles of stem cell quiescence, replication-associated DNA damage, telomere shortening, epigenetic alterations, and metabolic challenges as determinants of stem cell mutations and clonal dominance in aging

A. Minimum tillage investigations. B. Evaluation of stubble management techniques

77WH17 Continuous cropping site, Wongan Hills Research Station 77WH18 Rotational site, Wongan Hills Research Station 77M13 Heavy land continuous cropping site, Merredin Research Station 78M25 Light land continuous cropping site, Merredin Research Station 77M56 Rotational site, Merredin Research Station 77A16 Continuous cropping site, Avondale Research Station 77A43 Rotational site, Avondale Research Station 77E52 Rotational site, Esperance Downs Research Station 78BA42 Continuous Cropping Site, Badgingarra Research Station 77MT15 Continuous Cropping Site, Mt Barker Research Station 79WH6, Evaluation of stubble management practices, Wongan Hills Research Station 79M6 Heavy Land, Merredin Research Station 79M7 Light land, Merredin Research Statio

Atmospheric environment for space shuttle (STS-1) launch

Atmospheric conditions near space shuttle STS-1 launch time on April, 12, 1981, at Kennedy Space Center, Florida, are reported. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is presented. Also presented are the wind and thermodynamic parameters measured at the surface and aloft in the SRB descent/impact ocean area. Final meteorological data tapes for STS-1 vehicle ascent, and SRB descent have been constructed which consist of wind and thermodynamic parameters versus altitude

COMMERCIAL BEEF HERD REPLACEMENT STRATEGIES

Annualized cow ownership costs represent a large component of the total costs in a cow-calf enterprise, and therefore impact profitability. Annualized cow costs are determined in large part by the price or cost of that cow when it entered the herd. We find that ownership costs, and in turn cow-herd profitability, can be significantly impacted by heifer replacement strategies. Timing (within the cattle cycle) and method (raise vs. purchase) are both important considerations.Livestock Production/Industries,

An Analysis of Putative Roles for the CCR4-NOT Deadenylase-Complex Subunit Regena (NOT2) in microRNA-Mediated Gene Silencing in \u3cem\u3eDrosophila Melanogaster\u3c/em\u3e

microRNAs (miRNAs) are one class of small non-coding ribonucleic acid (RNA) molecules essential to development and homeostasis in plants and animals. miRNAs silence gene expression through complementary base pairing with target gene messenger RNAs and association with the miRNA-induced silencing complex (miRISC). The identification and characterization of cellular factors required for miRNA-mediated gene silencing is incomplete. A forward genetic screen was carried out in Drosophila melanogaster to generate flies defective for gene silencing. Silencing was assayed by expression of a Green Fluorescent Protein (GFP) reporter fused to the Brd gene 3’ UTR, which is regulated by miRNAs. Genetic analysis revealed that the CCR4-NOT deadenylase-complex subunit Regena (NOT2) is required for miRNA-mediated silencing of the reporter. In addition, perturbation of Regena function altered Drosophila eye development and resulting adult eye morphology. miRNAs are thought to silence target gene expression through a combination of translational repression and target mRNA degradation, though the detailed mechanism of this process is a matter of controversy. Novel genetic reagents to explore miRNA function in vivo have been generated and characterized. Ongoing efforts aim to explore whether Regena is required to silence other miRNA targets in vivo, and whether Regena is required for miRNA-mediated gene silencing at different stages of the Drosophila life cycle. Elucidation of the lesion in the Regena (NOT2) gene and the molecular nature of GFP reporter silencing will contribute to an understanding of the mechanism of miRNA-mediated gene silencing in vivo

Spectrographic Techniques and Analyses of Pine Needles

Author Institution: Crops Research Division, ARS, U. S. Dept. of Agriculture, and the Ohio Agriculture Experiment Station, Wooste

Atmospheric environment for Space Shuttle (STS-27) launch

Selected articles on atmospheric conditions observed near Space Shuttle STS-27 launch time on December 2, 1988, at Kennedy Space Center, Florida are summarized. STS-27 carried a Department of Defense payload and the flight azimuth in this report will be denoted by reference flight azimuth, since the actual flight azimuth is not known. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of pre-launch Jimsphere-measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-27 vehicle ascent was constructed. The STS-27 ascent atmospheric data tape was constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in post-flight performance assessments